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Based on sequence similarity, two additional potential family members, monooxygenase X and dopamine-β-hydroxylase-L, have been identified; their substrate specificity has not been determined. However, of the amino acid residues that are conserved among species, site-directed mutagenesis has identified a subset that are candidates for involvement in maintaining the structure of PAL and additional residues that are candidates for involvement in the PAL catalytic mechanism.


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